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Updated: Jun 30, 2026

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
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Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

Published on: March 22, 2016

Flanking-sequence exponential anchored-polymerase chain reaction amplification: a sensitive and highly specific

M A Pule1, A Rousseau, J Vera

  • 1Center for Cell and Gene Therapy, Baylor College of Medicine, Houston, TX, USA. mpule@ucl.ac.uk

Cytotherapy
|September 30, 2008
PubMed
Summary

We developed a new method, FLEA-PCR, to analyze retroviral DNA integration sites. This technique simplifies tracking cell fate and identifying potentially oncogenic integrations, improving stem cell research and therapy.

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