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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...

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Updated: Jun 30, 2026

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
08:43

Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model

Published on: February 7, 2018

Bioreactor maintained living skin matrix.

Mitchell R Ladd1, Sang Jin Lee, Anthony Atala

  • 1Wake Forest Institute for Regenerative Medicine, Wake Forest University Health Sciences, Winston-Salem, North Carolina, USA.

Tissue Engineering. Part A
|September 30, 2008
PubMed
Summary
This summary is machine-generated.

Researchers expanded human skin in a bioreactor, increasing its surface area by over 110% while maintaining viability. This novel method offers a promising solution for generating ample skin grafts for reconstructive surgery.

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Area of Science:

  • Tissue engineering
  • Regenerative medicine
  • Biotechnology

Background:

  • Skin grafting is crucial for reconstructive surgery, but donor site limitations hinder large wound coverage.
  • Existing methods like in vivo tissue expanders have significant drawbacks.
  • There is a need for methods to expand skin surface area in vitro while preserving tissue viability.

Purpose of the Study:

  • To investigate the feasibility of expanding human skin surface area in vitro using a computer-controlled bioreactor.
  • To assess the impact of in vitro expansion on skin viability, morphology, and mechanical properties.

Main Methods:

  • Human foreskin was cultured and incrementally expanded in a computer-controlled bioreactor over six days.
  • Evaluated morphological, ultrastructural, and mechanical properties using histology, scanning electron microscopy, mercury porosimetry, and tensile testing.

Main Results:

  • Achieved a 110.7% increase in skin surface area with maintained cell viability and proliferative potential.
  • Dermal structural integrity was preserved, with increased pore diameter (64.49%).
  • Mechanical properties of the expanded skin were not adversely affected.

Conclusions:

  • Computer-controlled bioreactor system enables successful in vitro expansion of living skin matrices.
  • This technique can generate sufficient skin for reconstructive procedures, overcoming donor site limitations.
  • Offers a potential breakthrough for treating large wounds requiring skin grafts.