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Updated: Jun 30, 2026
Assays for Validating Histone Acetyltransferase Inhibitors
Published on: August 6, 2020
[Effects of trichostatin A on the interaction between HBx and histone deacetylase protein 1]
Ju-qiang Han1, Qi-nong Ye, Li-Hua Ding
1Institute of Hepatology, Beijing Military General Hospital, Beijing 100700, China.
Objectives:
To study the effects of trichostatin A (TSA) on protein-protein interaction between HBx and histone deacetylase protein 1 (HDAC1).
Methods:
Both HBx and HDAC1 expressing vectors were constructed by the method of routine molecular cloning. The expression of HBx and HDAC1 were observed by Western blot assay. The protein-protein interaction was tested between HBx and HDAC1 by GST pull-down in vitro as well as co-immunoprecipitation in vivo.
Results:
Both HBx and HDAC1 expressing vectors were successfully constructed. Protein-protein interaction between HBx and HDAC1 existed both in vitro and in vivo. TSA, an inhibitor of HDAC1, had no effect on the interaction between HBx and HDAC1.
Conclusions:
HBx interacts with HDAC1 in vivo and in vitro in a non- TSA dependent way.
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