A novel and rapid method for determining integration frequency catalyzed by integron integrase intI1
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Zehua Yang1, Xiaofei Jiang, Quhao Wei
1Center of Laboratory Medicine, Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai 200040, China.
Journal of Microbiological Methods
|September 30, 2008
Abstract:
We developed a faster and more convenient method to determine integration frequency mediated by integron integrase intI1. This method based on real-time fluorescent quantitative PCR. By using this method, we revealed that the integration frequency of aadA2 gene cassette was 1.87x10(-4) when integrase intI1 was present, and the background frequency was less than 5.23x10(-8) without integrase intI1.
