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Updated: Jun 30, 2026

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach
12:32

Quantification of Plasmid-Mediated Antibiotic Resistance in an Experimental Evolution Approach

Published on: December 14, 2019

[A novel Salmonella Typhimurium plasmid, pAnkS: an example for plasmid evolution in antibiotic resistance]

Fikret Sahin1, Djursun Karasartova, Devran Gerçeker

  • 1Ankara Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Ankara. fsahin29@hotmail.com

Mikrobiyoloji Bulteni
|October 1, 2008
PubMed

Insights

A novel plasmid, pAnkS, was sequenced from Salmonella Typhimurium, revealing its ampicillin resistance gene and evolutionary link between known plasmids. This finding aids understanding of plasmid evolution and antibiotic resistance.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Context:

  • Antibiotic resistance in Salmonella Typhimurium is a significant public health concern.
  • Plasmids play a crucial role in the dissemination of antimicrobial resistance genes.
  • Understanding plasmid evolution is key to combating resistance spread.

Purpose:

  • To fully sequence and characterize a novel plasmid (pAnkS) from a multidrug-resistant Salmonella Typhimurium clinical isolate.
  • To analyze the genetic structure of pAnkS, including transposons and resistance genes.
  • To investigate the evolutionary relationship of pAnkS with other known plasmids.

Summary:

  • A 8271 bp plasmid, named pAnkS, was isolated from Salmonella Typhimurium and completely sequenced.
  • pAnkS contains an ampicillin resistance gene (blaTEM) within a Tn3-like transposon and shares a core region with EHEC plasmid p4821.
  • The plasmid shows homology to Salmonella plasmid pNTP16, suggesting pAnkS is an intermediate in plasmid evolution.

Impact:

  • Provides a complete genome sequence for a plasmid from Salmonella Typhimurium.
  • Elucidates the genetic basis of ampicillin resistance in the studied isolate.
  • Offers insights into the evolutionary pathways of plasmids, contributing to antimicrobial resistance research.

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