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Updated: Jun 30, 2026

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
Published on: October 17, 2013
[Advances in the MDCK-MDR1 cell model and its applications to screen drug permeability]
Abstract:
This article introduces the characteristics and establishment of MDCK-MDR1 cell line, and its applications as an in vitro model for the assessment of the membrane permeability properties of drugs. MDCK-MDR1 cell model is widely used in drug screenings, and the study of mechanisms of drug interaction and absorption or transport. The review summarizes the progress and applications of MDCK-MDR1 cell model. Because there is high polarized P-glycoprotein (P-gp) expression in MDCK-MDR1 cells, it could be used as a drug transport model to screen P-gp substrates or inhibitors, as well as models of intestinal mucosa, blood brain barrier and kidney. Although there are some disadvantages in MDCK-MDR1 cell model, it is an effective tool for implementing permeability of drug in an optimal fashion.
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