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Updated: Jun 30, 2026
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Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants
Published on: January 26, 2019
Human amnion-derived mesenchymal stem cells are a potential source for uterine stem cell therapy
1Department of Biomedical Science and Technology, Research Center for Drugs, Institute of Biomedical Science and Technology, Konkuk University, Seoul, South Korea.
Cell Proliferation
|October 1, 2008
Summary
Human amnion-derived mesenchymal (HAM) cells show potential as a therapeutic stem cell source. These cells express key markers and can be tracked in vivo after transplantation, indicating therapeutic promise.
Area of Science:
- Stem Cell Biology
- Regenerative Medicine
- Gynecology
Background:
- Human amnion is an accessible source of mesenchymal stem cells (MSCs).
- Human amnion-derived mesenchymal (HAM) cells exhibit MSC-like characteristics.
- Ethical considerations are minimized with amnion-derived cells.
Purpose of the Study:
- To further characterize HAM cells for in vivo therapeutic applications.
- To assess the therapeutic potential of HAM cells.
Main Methods:
- Flow cytometry to analyze cell surface markers (e.g., CD90, CD105).
- Karyotyping to confirm normal chromosome structure.
- In vivo tracking of enhanced green fluorescence protein (EGFP)-expressing HAM cells in mice via intrauterine and intravenous administration.
Main Results:
- HAM cells express MSC markers (CD90, CD105) and HOXA genes (HOXA9, HOXA10, HOXA11).
- HAM cells maintain a normal karyotype at the 10th passage.
- EGFP-expressing HAM cells were detected in mouse uterine mesenchyme and lungs post-infusion/injection.
Conclusions:
- HAM cells possess characteristics of mesenchymal stem cells.
- HAM cells demonstrate potential for in vivo therapeutic applications.
- Human amnion is a promising source for therapeutic stem cells.
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