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Validation of a liquid chromatography-tandem mass spectrometry method to enable quantification of 3-iodothyronamine
Andrea E DeBarber1, Travis Geraci, Vincent P Colasurdo
1Department of Physiology and Pharmacology, Oregon Health and Science University, Portland, OR 97239, USA.
Abstract:
There is great interest lately in the availability of analytical methods for quantification of 3-iodothyronamine from blood and tissues. To date, no validated method for determination of 3-iodothyronamine from biological matrices has been described. Detailed in this report is an LC-MS/MS method that permits accurate and reproducible quantification of pharmacological concentrations of 3-iodothyronamine from rat serum, with a 0.0008 M lower limit of quantification. Endogenous 3-iodothyronamine was observed from rodent and human serum (0.2 mL) at the method limit of detection. In summary, the LC-MS/MS method enables quantification of circulating 3-iodothyronamine to allow examination of a relationship with biological activity.
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