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Analysis of urinary andrographolides and antioxidant status after oral administration of Andrographis paniculata leaf
G A Akowuah1, I Zhari, A Mariam
1University College Sedaya International, Taman Connaught, 56000 Cheras, Kuala Lumpur, Malaysia. wuahmy@yahoo.com
Abstract:
A simple high-performance liquid chromatography (HPLC) method was developed to determine the content of andrographolide (AP) and 14-deoxy-11,12-dideoxyandrographolide (DIAP) in a pooled urine of rat obtained within 24h after an oral dose of Andrographis paniculata leaf extract at 1g/kg body weight. Cumulative urinary excretion of AP and DIAP in 24h after oral administration of the extract was 0.88% and 1.61% of oral dose administered, respectively. The extract showed significant reduction (p<0.05) of MDA levels and elevation of total antioxidant status in rat urine samples collected in 24 after oral administration.