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Updated: Jun 30, 2026

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

Complex ciprofloxacin resistome revealed by screening a Pseudomonas aeruginosa mutant library for altered

Elena B M Breidenstein1, Bhavjinder K Khaira, Irith Wiegand

  • 1Centre for Microbial Diseases and Immunity Research, University of British Columbia, Room 232, 2259 Lower Mall, Lower Mall Research Station, Vancouver, British Columbia V6T1Z4, Canada.

Antimicrobial Agents and Chemotherapy
|October 1, 2008
PubMed

Insights

This study screened a Pseudomonas aeruginosa mutant library to understand antibiotic resistance. Researchers identified numerous mutants with altered susceptibility to ciprofloxacin, revealing key genes involved in resistance mechanisms.

Area of Science:

  • Microbiology
  • Genetics
  • Pharmacology

Background:

  • Pseudomonas aeruginosa presents significant therapeutic challenges due to inherent and acquired antibiotic resistance.
  • Fluoroquinolones like ciprofloxacin are critical antibiotics, but resistance limits their efficacy.

Purpose of the Study:

  • To identify genes contributing to intrinsic and mutational resistance in Pseudomonas aeruginosa.
  • To understand the genetic basis of fluoroquinolone resistance in this opportunistic pathogen.

Main Methods:

  • Screening of the comprehensive PA14 mutant library of Pseudomonas aeruginosa.
  • Assessing changes in susceptibility (increased or decreased) to ciprofloxacin using broth dilution assays.

Main Results:

  • Identified 35 mutants with increased susceptibility, highlighting genes involved in intrinsic resistance.
  • Identified 79 mutants with decreased susceptibility, indicating genes related to mutational resistance.
  • Confirmed findings through rigorous broth dilution testing.

Conclusions:

  • The study successfully identified genetic determinants of ciprofloxacin resistance in Pseudomonas aeruginosa.
  • Findings provide valuable insights into Pseudomonas aeruginosa's resistance mechanisms, aiding in the development of new therapeutic strategies.

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