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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Related Experiment Video

Updated: Jun 30, 2026

Fluorescence Imaging with One-nanometer Accuracy (FIONA)
11:56

Fluorescence Imaging with One-nanometer Accuracy (FIONA)

Published on: September 26, 2014

Entangled-photon coincidence fluorescence imaging.

Giuliano Scarcelli1, Seok H Yun

  • 1Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, 50 Blossom St., Boston, MA 02114, USA.

Optics Express
|October 1, 2008
PubMed
Summary

This study introduces fluorescence imaging with entangled photon pairs. This technique uses one photon to locate fluorescence, preventing blur from scattered light for clearer images.

Area of Science:

  • Quantum optics
  • Biomedical imaging
  • Photonics

Background:

  • Fluorescence imaging is crucial for biological and medical research.
  • Traditional methods face challenges with image blur caused by scattered photons.
  • Entangled photons offer novel possibilities for advanced imaging techniques.

Purpose of the Study:

  • To develop a novel fluorescence imaging method utilizing the quantum correlations of entangled photon pairs.
  • To overcome the limitations of image blur caused by multiply-scattered photons in conventional fluorescence microscopy.
  • To demonstrate the feasibility of using entangled photons for high-resolution fluorescence imaging.

Main Methods:

  • Employing entangled photon pairs where one photon acts as a probe and the other as a detector.

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  • Leveraging the second-order correlation function of entangled photons to determine fluorescence origin.
  • Utilizing the property that fluorescent molecules act as decoherence sources, breaking entanglement and isolating the fluorescence signal.

    • Main Results:

    • Demonstrated a fluorescence imaging technique based on quantum correlations.
    • Showed that multiply-scattered fluorescence photons do not degrade image quality due to entanglement breaking.
    • Established a method to pinpoint fluorescence absorption sites with enhanced clarity.

    Conclusions:

    • The proposed method offers a pathway to reduce image blur in fluorescence imaging.
    • Entangled photon pairs provide a unique tool for high-fidelity optical measurements.
    • This quantum-enhanced imaging approach has potential applications in various scientific fields requiring precise visualization.