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Updated: Jun 30, 2026

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

Published on: February 12, 2018

Plastinated tissue samples as three-dimensional models for optical instrument characterization.

Daniel L Marks¹, Eric J Chaney, Stephen A Boppart

¹Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 N. Mathews Ave., Urbana, IL 61801, USA.

|October 1, 2008

Summary

This summary is machine-generated.

Plastination transforms biological tissues into durable 3D models for advanced optical imaging. This technique preserves intricate structures, enabling detailed characterization with instruments like optical coherence tomography (OCT).

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Fabrication and Characterization of Optical Tissue Phantoms Containing Macrostructure

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Published on: February 12, 2018

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Staining and High-Resolution Imaging of Three-Dimensional Organoid and Spheroid Models

Published on: March 27, 2021

Area of Science:

Biomedical Engineering
Optical Imaging Technologies
Histopathology

Background:

Traditional histology is limited to 2D due to sample sectioning.
Emerging 3D optical imaging (OCT, DOT, MPM) requires minimally disruptive tissue preparation methods.

Purpose of the Study:

To propose and evaluate plastination as a method for creating 3D tissue models for optical instrument characterization.
To demonstrate the utility of plastinated tissues for 3D structural investigation and instrument performance testing.

Main Methods:

Tissues are processed using plastination, involving infusion with transparent polymers.
Plastinated samples are rendered safe for handling and long-term preservation.
Optical coherence tomography (OCT) was used to image plastinated rat tissues.

Main Results:

Plastination successfully created stable, transparent 3D tissue models.
OCT imaging revealed detailed three-dimensional structures within the plastinated samples.
The method facilitates investigation of properties lost in conventional 2D histology.

Conclusions:

Plastination is a viable technique for preparing 3D biological specimens for advanced optical analysis.
These 3D models are valuable for calibrating and comparing optical imaging instruments.
The approach opens new avenues for studying tissue properties in their native 3D context.