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Updated: Jun 29, 2026
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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study
Published on: August 16, 2019
Affinity chromatography: history, perspectives, limitations and prospects.
Ana Cecília A Roque1, Christopher R Lowe
1Faculdade de Ciéncias e Tecnologia, Universidade Nova de Lisboa, Portugal.
Methods in Molecular Biology (Clifton, N.J.)
|October 2, 2008
Summary
Affinity chromatography offers efficient biomolecule isolation in biotechnology. This review explores its history, current use, limitations, and future potential for purifying biological molecules.
Area of Science:
- Biotechnology
- Biochemistry
- Separation Science
Background:
- Biomolecule separation and purification traditionally relied on empirical methods.
- Adsorption chromatography, specifically affinity chromatography, has emerged as a key technique.
- This method utilizes specific biological affinity for targeted molecule isolation.
Observation:
- Affinity chromatography leverages adsorbent materials with specific binding properties.
- The technique is highly effective for isolating complex biomolecules.
- Its application is crucial in modern biotechnology workflows.
Findings:
- This review provides a historical overview of affinity chromatography.
- It details the current status and inherent limitations of the technique.
- Future prospects for affinity chromatography in biotechnology are discussed.
Implications:
- Understanding the evolution and capabilities of affinity chromatography is vital for biotechnological advancements.
- The technique's efficiency impacts drug discovery, diagnostics, and bioprocessing.
- Further development could overcome current limitations, expanding its utility.
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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
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