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Updated: Jun 29, 2026
Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
Published on: February 25, 2021
[Isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans]
Yuan-Hui Deng1, Hai-Lan Wang, Ling Han
1The Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120, China. yhdeng62@126.com
Objective:
To study the methods of extraction, isolation, purification and biological activities of Arnebia euchroma glycosaminoglycans (AEG).
Methods:
AEG was purified by distilled water extraction, ethanol fractionation, Sephadex column chromatography. The purity and molecular weight and concentration of AEG were measured by HPLC; We divided the experiment into Physiological Saline group and the other eight groups with different doses of AEG, established RT-PRC method to observe the anti-HPV effect after their actions on the verruca tissues.
Results:
Using HPLC, the group of AEG was divided into two glycosaminoglycans with different molecular weight as 27336 and 1152. Bioassay results showed that AEG had anti-HPV-DNA activity, the lowest effective concentration was 0.781 mg/ml.
Conclusion:
AEG extracted by this method is a mixture with two molecular weight glycosaminoglycans, and has obvious anti-HPV effect.
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