Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Simplification of a Three-Constant Intraocular Lens Calculation Formula to a Single-Constant Approach: The Haigis Formula.

Diagnostics (Basel, Switzerland)·2026
Same author

Machine Learning-Based Prediction of Long-Term Intraocular Pressure Fluctuations in Open-Angle Glaucoma.

Ophthalmology science·2026
Same author

Intraocular Lens Calculation Concept Based on Aphakic Refraction-Considerations on a Cornea Model With Two Refracting Surfaces.

Ophthalmic & physiological optics : the journal of the British College of Ophthalmic Opticians (Optometrists)·2026
Same author

Refractive and Corneal Astigmatism After Implantation of a Supraciliary Drainage Device in Microinvasive Glaucoma Surgery.

Journal of refractive surgery (Thorofare, N.J. : 1995)·2026
Same author

Correction: Prediction of the ectasia screening index from raw Casia2 volume data for keratoconus identification by using convolutional neural networks.

PloS one·2026
Same author

Response to the Letter to the Editor: Dual-Zone Keratometry for Identifying Central Radius and Corneal Asphericity.

Current eye research·2026
Same journal

Limitations of Mono-Exponential Individual Fitting as a Reference Model for Single-Time-Point Dosimetry in [<sup>177</sup>Lu]Lu-PSMA-617 Therapy.

Zeitschrift fur medizinische Physik·2026
Same journal

Determination of long-range resistance from high resolution impedance spectroscopy in human cadaveric heads.

Zeitschrift fur medizinische Physik·2026
Same journal

Patient-specific quality assurance in stereotactic radiotherapy: clinical practice in absence of guidelines - status and new approach from the DGMP working group for physics and technology in stereotactic radiotherapy.

Zeitschrift fur medizinische Physik·2026
Same journal

Potential and challenges of Positron Emission Tomography beyond conventional preclinical and clinical imaging.

Zeitschrift fur medizinische Physik·2026
Same journal

Evaluation of PET/CT Artificial Intelligence Image Reconstructions VS Harmonized Clinical Reconstruction.

Zeitschrift fur medizinische Physik·2026
Same journal

Adaptation of quality control pipeline for Skeletal Muscle 31P MR Spectroscopy at 3T and 7T.

Zeitschrift fur medizinische Physik·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Linear method of fluorescent source reconstruction in a diffusion medium.

Edgar Janunts1, Thomas Pöschinger, Holger Brünner

  • 1Medical Optics at the Institute of Medical Physics, University of Erlangen-Nuremberg, Henkestrasse 91, D-91052 Erlangen. edgar.janunts@imp.uni-erlangen.de

Zeitschrift Fur Medizinische Physik
|October 2, 2008
PubMed
Summary
This summary is machine-generated.

This study presents a new 2D reconstruction method for fluorescent sources within diffusion media. The technique uses surface light measurements and solves an inverse problem for accurate imaging.

More Related Videos

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Related Experiment Videos

Last Updated: Jun 29, 2026

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
15:10

From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope

Published on: October 9, 2014

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
12:06

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Published on: February 1, 2017

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
05:56

Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells

Published on: November 12, 2020

Area of Science:

  • Biomedical Optics
  • Photon Transport Imaging
  • Fluorescence Imaging

Background:

  • Accurate localization of fluorescent sources in scattering media is crucial for various applications.
  • Existing methods often face challenges with signal attenuation and scattering.
  • Developing robust imaging techniques for turbid media remains an active research area.

Purpose of the Study:

  • To introduce a novel 2D reconstruction method for fluorescent source distributions.
  • To enable imaging of fluorescence within diffusion media using surface measurements.
  • To validate the method's applicability through experimental examples.

Main Methods:

  • Utilizing planar measurements of emission light at the surface after plane wave excitation.
  • Employing the diffusion approximation of the radiative transport equation (RTE) for photon transport modeling.
  • Solving a linear inverse source problem for image reconstruction.
  • Applying least squares minimization for optimization.

Main Results:

  • Successful 2D reconstruction of fluorescent source distributions was demonstrated.
  • The method accurately images sources within a homogeneous diffusion medium.
  • The theoretical approach showed applicability in representative working examples.

Conclusions:

  • The developed method provides a viable approach for 2D fluorescence source reconstruction.
  • This technique enhances imaging capabilities in scattering biological tissues.
  • Further applications in areas like medical diagnostics and research are anticipated.