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Updated: Jun 29, 2026

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
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Isomerization in fluorescent protein chromophores involves addition/elimination

Jian Dong1, Fardokht Abulwerdi, Anthony Baldridge

  • 1School of Chemistry and Biochemistry, 901 Atlantic Drive; Georgia Institute of Technology, Atlanta, Georgia 30332-0400, USA.

Journal of the American Chemical Society
|October 2, 2008
PubMed
Summary

The green fluorescent protein (GFP) chromophore’s thermal reversion is surprisingly fast due to a novel nucleophilic addition/elimination mechanism, not just low activation energy. This finding impacts understanding of fluorescent protein function and blinking.

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