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Related Concept Videos

The Extracellular Matrix01:29

The Extracellular Matrix

Overview
In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.
Composition of the Extracellular Matrix
The extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse...
The Extracellular Matrix01:42

The Extracellular Matrix

In order to maintain tissue organization, many animal cells are surrounded by structural molecules that make up the extracellular matrix (ECM). Together, the molecules in the ECM maintain the structural integrity of tissue as well as the remarkable specific properties of certain tissues.Composition of the Extracellular MatrixThe extracellular matrix (ECM) is commonly composed of ground substance, a gel-like fluid, fibrous components, and many structurally and functionally diverse molecules.
Extracellular Matrix01:26

Extracellular Matrix

Unlike epithelial tissue, which is composed of cells closely packed with little or no extracellular space in between, connective tissue cells are dispersed in a matrix. This extracellular matrix (ECM) is composed of fibrous proteins like collagen, elastin, and fibronectin in a ground substance consisting of interstitial fluid, cell adhesion proteins, and proteoglycans. The proteoglycans form a gel-like material in the spaces between cells and provide hydration, buffering, binding, and force...
Cell-matrix's Response to Mechanical Forces01:13

Cell-matrix's Response to Mechanical Forces

In animal cells, the extracellular matrix allows cells within tissues to withstand external stresses and transmits signals from the outside of the cell to the inside. The extracellular matrix is extensive, and its composition varies between different types of tissues. For example, the reticular fibers and ground substance make up the ECM in loose connective tissue, while collagen and bone minerals make up the ECM of bone tissue. 
Anchoring junctions mechanically attach a cell to the...
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...

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Related Experiment Video

Updated: Jun 29, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

Motif-programmed artificial extracellular matrix.

Katsutoshi Kokubun1, Kenji Kashiwagi, Masao Yoshinari

  • 1Department of Clinical Pathophysiology, Division of Oral Implants Research and Oral Health Science Center, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba, 261-8501 Japan.

Biomacromolecules
|October 2, 2008
PubMed
Summary
This summary is machine-generated.

Researchers created artificial proteins by combining cell attachment and titanium-binding motifs. These proteins bind titanium and cells, acting as an artificial extracellular matrix for tissue engineering and regenerative medicine applications.

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Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
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Last Updated: Jun 29, 2026

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering
10:30

Design and Construction of Artificial Extracellular Matrix (aECM) Proteins from Escherichia coli for Skin Tissue Engineering

Published on: June 11, 2015

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins
09:49

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
06:01

Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

Published on: February 2, 2019

Area of Science:

  • Biotechnology
  • Materials Science
  • Tissue Engineering

Background:

  • Motif-programming enables artificial protein creation by combining functional peptide motifs.
  • This method is ideal for developing liaison molecules between cells and inorganic materials.

Purpose of the Study:

  • To create artificial proteins by programming cell attachment (RGD) and titanium-binding (minTBP-1) motifs.
  • To evaluate the proteins' ability to bind titanium and osteoblast-like cells.
  • To assess their potential as an artificial extracellular matrix on titanium materials.

Main Methods:

  • Utilized motif-programming to combine a natural cell attachment motif (RGD) and an artificial titanium-binding motif (minTBP-1).
  • Tested the binding affinity of the created artificial proteins to titanium and MC3T3-E1 osteoblast-like cells.

Main Results:

  • The artificial proteins demonstrated reversible binding to titanium.
  • The proteins successfully bound MC3T3-E1 osteoblast-like cells.
  • The proteins mimicked fibronectin functions, serving as an artificial extracellular matrix on titanium.

Conclusions:

  • The motif-programming system successfully created artificial proteins with dual functionality.
  • These artificial proteins can interface with both inorganic materials (titanium) and biological cells.
  • The system holds significant potential for applications in tissue engineering and regenerative medicine.