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Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...

Non-Canonical Wnt Signaling Pathways

Non-Canonical Wnt Signaling Pathways

Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...

Non-Canonical Wnt Signaling Pathways

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Eukaryotic Transcription Inhibitors

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Related Experiment Video

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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

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A Wnt-fall for gene regulation: repression.

Nate P Hoverter¹, Marian L Waterman

¹Department of Microbiology and Molecular Genetics, University of California, Irvine, Irvine, CA 92697, USA.

Science Signaling

|October 2, 2008

Summary

The Wnt signaling pathway

Area of Science:

Molecular Biology
Gene Regulation
Cell Signaling

Background:

Canonical Wnt signaling pathway regulates gene transcription via lymphoid enhancer factor (LEF) and T cell factor (TCF) transcription factors.
The LEF/TCF:beta-catenin complex typically activates gene transcription, but can also repress it.
Previous research has explored LEF/TCF:beta-catenin-mediated transcriptional activation and repression.

Purpose of the Study:

To investigate a novel mechanism of Wnt target gene repression.
To identify new DNA elements recognized by TCF transcription factors.
To elucidate the role of beta-catenin as a transcriptional repressor in Wnt signaling.

Main Methods:

Analysis of Wnt target gene transcription.
Identification and characterization of novel DNA-binding elements.

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Related Experiment Videos

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Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Application of RNAi and Heat-shock-induced Transcription Factor Expression to Reprogram Germ Cells to Neurons in C. elegans

Published on: January 1, 2018

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

Biochemical assays to study protein-DNA interactions.

Reporter gene assays to assess transcriptional activity.

Main Results:

A novel DNA element recognized by TCF was identified.
This novel DNA element mediates the repression of Wnt target genes.
Beta-catenin was shown to function as a direct transcriptional repressor through this mechanism.

Conclusions:

A new mode of Wnt target gene repression has been uncovered.
TCF binding to a specific DNA element recruits beta-catenin as a repressor.
This finding expands the understanding of Wnt pathway regulation beyond transcriptional activation.