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Updated: Jun 29, 2026
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An Adoptive Transfer Model of Rheumatoid Arthritis in Mice
Published on: June 6, 2025
Strain and model dependent differences in inflammatory cell recruitment in mice
J L Hoover-Plow1, Y Gong, A Shchurin
1Department of Cardiovascular Medicine and Department of Molecular Cardiology, Joseph J. Jacobs Center for Thrombosis and Vascular Biology, NB50, Cleveland Clinic Lerner Research Institute, Cleveland, OH 44195, USA. hooverj@ccf.org
Summary
Genetic differences in mouse strains significantly impact inflammatory responses, affecting leukocyte recruitment and cytokine profiles. These variations are stimulus-specific, influencing disease susceptibility.
Area of Science:
- Immunology
- Genetics
Background:
- Inbred mouse strains exhibit distinct genetic backgrounds.
- Understanding genetic influences on inflammation is crucial for disease research.
Purpose of the Study:
- To investigate genetic variations in inflammatory responses among A/J, 129/svJ, and C57BL/6J mouse strains.
- To compare leukocyte recruitment and mediator profiles under different inflammatory stimuli.
Main Methods:
- Inflammatory stimuli included thioglycollate and biomaterial implants.
- Quantified peritoneal leukocyte recruitment (neutrophils, macrophages).
- Measured matrix metalloproteinases (MMPs) and cytokines in lavage fluid.
Main Results:
- A/J and 129/svJ strains showed reduced neutrophil and macrophage recruitment compared to C57BL/6J in the thioglycollate model.
- 129/svJ exhibited increased leukocyte recruitment with elevated cytokines (MIP-2, MCP-1) in the biomaterial implant model.
- Reduced leukocyte influx correlated with lower MMP-9 levels in the thioglycollate model.
Conclusions:
- Leukocyte recruitment and inflammatory mediator profiles are genetically determined and strain-specific.
- Responses to inflammatory stimuli are model-dependent, highlighting stimulus specificity.
- Genetic control of inflammatory cell recruitment may influence susceptibility to complex diseases.
