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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Jun 29, 2026

Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Making Patch-pipettes and Sharp Electrodes with a Programmable Puller

Published on: October 8, 2008

Flip-the-tip: automated patch clamping based on glass electrodes.

Michael Fejtl1, Uwe Czubayko, Alexander Hümmer

  • 1Flyion GmbH, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|October 2, 2008
PubMed
Summary

This study introduces the FlipTip system for automated patch-clamp electrophysiology, enabling high-throughput ion channel analysis. The automated robot achieves hundreds of data points daily, accelerating drug discovery.

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Area of Science:

  • Electrophysiology
  • Ion Channel Research
  • Automated Systems

Background:

  • Patch-clamp electrophysiology is crucial for studying ion channel function.
  • Traditional methods are often labor-intensive and low-throughput.
  • Need for automated solutions to increase data acquisition efficiency.

Purpose of the Study:

  • To present the novel FlipTip system for automated patch-clamp recordings.
  • To demonstrate the system's capability for high-throughput ion channel measurements.
  • To evaluate the efficiency and reliability of the automated platform.

Main Methods:

  • Utilized a FlipTip system with borosilicate glass pipettes and a liquid handler.
  • Employed continuous suction to form GigaSeal and establish whole-cell or perforated patch configurations.
  • Integrated automated compound delivery and voltage-clamp measurements.
  • Operated the Flyscreen 8500 automated patch-clamp robot for unattended experiments.

Main Results:

  • Achieved classical GigaSeal (1-5 GΩ) formation consistently.
  • Enabled simultaneous ion channel response measurements in multiple sockets.
  • Demonstrated high data throughput, generating hundreds of data points per day.
  • Successfully performed both whole-cell and perforated patch configurations.

Conclusions:

  • The FlipTip system offers an efficient and automated approach to patch-clamp electrophysiology.
  • This automated platform significantly enhances throughput for ion channel screening.
  • The system is suitable for both standard and perforated patch-clamp techniques, accelerating research and drug discovery.