Related Experiment Video
Updated: Jun 29, 2026
10:34
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
Published on: April 23, 2017
Adhesive substrate-modulation of adaptive immune responses.
Abhinav P Acharya1, Natalia V Dolgova, Michael J Clare-Salzler
1Materials Science and Engineering, University of Florida, P.O. Box 116400, Gainesville, Florida 32611-6400, USA. abhinav9@ufl.edu
Biomaterials
|October 3, 2008
Summary
Adhesive substrates differentially modulate dendritic cell (DC) maturation and cytokine production. Substrate choice impacts DC cytokine profiles, influencing T-helper cell responses and T(h)1 cell differentiation.
Area of Science:
- Biomaterials Science
- Immunology
- Cell Biology
Background:
- Adsorbed proteins on biomaterials influence inflammatory responses.
- Adhesion-dependent signaling in dendritic cells (DCs) is crucial for immune modulation but not fully understood.
Purpose of the Study:
- To investigate how adhesive substrates affect DC maturation and adaptive immune responses.
- To determine the substrate-dependent modulation of DC cytokine production and T-cell activation.
Main Methods:
- Culturing DCs on various adhesive substrates (collagen, vitronectin, albumin, serum-coated).
- Analyzing DC adhesion, morphology, and expression of stimulatory molecules.
- Quantifying pro-inflammatory (IL-12p40) and anti-inflammatory (IL-10) cytokine production.
- Assessing DC-mediated allogeneic CD4(+) T-cell proliferation and T-helper cell responses (Th1/Th2).
Main Results:
- Adhesive substrates support similar DC adhesion and stimulatory molecule expression.
- DC morphology and cytokine production (IL-12p40, IL-10) are substrate-dependent.
- Collagen and vitronectin substrates promote higher IL-12p40 production, while albumin and serum promote higher IL-10.
- Substrate-dependent IL-12p40 correlates with CD4(+) T-cell proliferation and Th1 responses (IFN-gamma).
- Substrate-dependent trends in Th2 responses (IL-4) were observed, independent of DC IL-10 production.
Conclusions:
- Adhesive substrate properties significantly dictate DC maturation and immune polarization.
- This understanding is vital for designing biomaterials for tissue engineering, vaccines, and immunotherapy.
- Tailoring biomaterial surfaces can control DC function for desired immune outcomes.
Related Concept Videos
01:40Cell-mediated Immune Responses
Overview
01:31Immunoglobulin-like Cell Adhesion Molecules
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
01:24B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
01:20Cell Adhesion Molecules - Types and Functions
Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily involved in a...
CAM Families
The Integrin family of proteins is primarily involved in a...
01:20Special Features of Adaptive Immunity
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...