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Updated: Jun 29, 2026

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
Published on: August 4, 2021
Pholasin luminescence is enhanced by addition of dehydrocoelenterazine
Masaki Kuse1, Eiko Tanaka, Toshio Nishikawa
1Chemical Instrument division, Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan. kuse@cic.nagoya-u.ac.jp
Abstract:
Pholasin is a bioluminescent photoprotein of Pholas dactylus. Pholasin is a commercially available photoprotein used to measure intracellular reactive oxygen species. Although extensive efforts have been carried out to determine the chemical structure of the prosthetic (chromophore) group, it still remains unclear to date. Herein, we report the enhancement of pholasin luminescence by the addition of dehydrocoelenterazine, which is organic substance of luminous squids' photoprotein.
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