Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Diphtheria01:28

Diphtheria

Diphtheria is an acute, toxin-mediated infectious disease that primarily affects the upper respiratory tract. It is caused by Corynebacterium diphtheriae, a Gram-positive, pleomorphic rod that lacks spore-forming capability and exhibits a characteristic club-shaped morphology under microscopic examination. While C. diphtheriae can asymptomatically colonize mucosal surfaces, clinical disease manifests only when the bacterial strain is lysogenized by a specific β-corynephage. This phage...
Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations01:15

Determination of Multiple Dosing Parameters: Steady-State, Minimum and Maximum Concentrations

Gentamicin, an aminoglycoside antibiotic, is commonly administered via intermittent intravenous infusion to treat severe infections. An intermittent one-hour infusion of gentamicin, administered at eight-hour intervals, allows for precise control of plasma drug concentrations, minimizing toxicity while ensuring therapeutic efficacy. Pharmacokinetic principles govern the dynamics of plasma concentrations and can be mathematically described using specific equations.The plasma drug concentration...
Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Preclinical Development: Overview01:28

Preclinical Development: Overview

Preclinical development consists of a series of tests that ensure the safety and efficacy of a new therapeutic compound before it is tested in humans. There are four main phases to this process. First, safety pharmacology tests are conducted to ensure the drug does not produce any acutely harmful effects. These tests examine parameters such as bronchoconstriction, cardiac dysrhythmias, blood pressure changes, and ataxia. Next, preliminary toxicological testing is performed to determine the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular characterization of plasmids encoding bla<sub>CTX-M</sub> from faecal Escherichia coli in travellers returning to the UK from South Asia.

The Journal of hospital infection·2021
Same author

Results from the first English stool bank using faecal microbiota transplant as a medicinal product for the treatment of <i>Clostridioides difficile</i> infection.

EClinicalMedicine·2020
Same author

Novel clinically relevant antibiotic resistance genes associated with sewage sludge and industrial waste streams revealed by functional metagenomic screening.

Environment international·2019
Same author

Prevalence of Listeria spp. on the Hands of Food Workers.

Journal of food protection·2019
Same author

Investigation of community carriage rates of Clostridium difficile and Hungatella hathewayi in healthy volunteers from four regions of England.

The Journal of hospital infection·2017
Same author

Correction for Cairns et al., "Comparative Genome Analysis and Global Phylogeny of the Toxin Variant Clostridium difficile PCR Ribotype 017 Reveals the Evolution of Two Independent Sublineages".

Journal of clinical microbiology·2017
Same journal

Emerging resistance in staphylococci following long-term dalbavancin treatment for prosthetic joint infections.

The Journal of antimicrobial chemotherapy·2026
Same journal

Microbiology testing around the time of antibiotic initiation among residents of long-term care facilities.

The Journal of antimicrobial chemotherapy·2026
Same journal

Insights into the mechanisms underlying cell wall-active agents and gentamicin bactericidal synergism against Enterococcus faecalis.

The Journal of antimicrobial chemotherapy·2026
Same journal

Re-evaluating the 14-day rule: short-course antifungal therapy for uncomplicated candidaemia in a multicentre cohort study.

The Journal of antimicrobial chemotherapy·2026
Same journal

Impact of selective digestive decontamination on the pangenome composition of ESBL-E. coli.

The Journal of antimicrobial chemotherapy·2026
Same journal

Compounds of the γ-carboline family inhibit trans-translation.

The Journal of antimicrobial chemotherapy·2026
See all related articles

Related Experiment Video

Updated: Jun 29, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

Pre-clinical experience with daptomycin.

P M Hawkey1

  • 1Division of Immunity and Infection, The Medical School, University of Birmingham, Birmingham, UK. peter.hawkey@heartofengland.nhs.uk

The Journal of Antimicrobial Chemotherapy
|October 10, 2008
PubMed
Summary
This summary is machine-generated.

Daptomycin is a potent bactericidal antibiotic for Gram-positive infections, but its effectiveness depends on calcium levels and it

Related Experiment Videos

Last Updated: Jun 29, 2026

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium
12:08

Rapid Antimicrobial Susceptibility Testing by Stimulated Raman Scattering Imaging of Deuterium Incorporation in a Single Bacterium

Published on: February 14, 2022

Area of Science:

  • Pharmacology
  • Microbiology
  • Infectious Diseases

Background:

  • Daptomycin is a broad-spectrum bactericidal antibiotic targeting Gram-positive bacteria via membrane depolarization.
  • Its in vitro activity is significantly influenced by calcium ion concentration.
  • Conventional disc diffusion testing is unreliable for daptomycin due to its high molecular weight and poor diffusion.

Purpose of the Study:

  • To provide guidance on daptomycin susceptibility testing.
  • To review daptomycin's pharmacokinetic and pharmacodynamic properties.
  • To outline current clinical applications and safety considerations for daptomycin.

Main Methods:

  • Minimum Inhibitory Concentration (MIC) determination in the presence of defined calcium concentrations is recommended.
  • Pharmacokinetic analysis including half-life, protein binding, and excretion routes.
  • Review of clinical data regarding efficacy, safety, and resistance patterns.

Main Results:

  • Daptomycin activity is concentration-dependent with a prolonged post-antibiotic effect.
  • Linear pharmacokinetics with an 8-9 hour half-life; renal excretion is primary.
  • Licensed for complicated skin/soft tissue infections, bacteremia, and endocarditis caused by Staphylococcus aureus.

Conclusions:

  • Daptomycin is a valuable agent for Gram-positive infections, offering an alternative to vancomycin.
  • Monitoring for daptomycin resistance is crucial for maintaining clinical utility.
  • Weekly creatinine phosphokinase monitoring is advised due to rare instances of myopathy.