Related Experiment Video
Updated: Jun 29, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope
Published on: April 7, 2014
Quantitative phase and refractive index analysis of optical fibers using differential interference contrast
Betty Kouskousis1, Daniel J Kitcher, Stephen Collins
1Optical Technology Research Laboratory, Centre for Telecommunications and Microelectronics, Victoria University, P.O. Box 14428, Melbourne, Victoria 8001, Australia. betty.kouskousis@live.vu.edu.au
Abstract:
A systematic and straightforward image processing method to extract quantitative phase and refractive index data from weak phase objects is presented, obtained using differential interference contrast (DIC) microscopy. The method is demonstrated on DIC images of optical fibers where a directional integration routine is applied to the DIC images to extract phase and refractive index information using the data obtained across the whole DIC image. By applying the inverse Abel transform to the resultant phase images, an accurate refractive index profile is obtained. The method presented here is compared to the refracted near-field technique, typically used to obtain the refractive index profile of optical fibers, and shows excellent agreement. It is concluded that through careful image processing procedures, DIC microscopy can be successfully implemented to obtain quantitative phase and refractive index information of optical fibers.
Related Concept Videos
Phase Contrast and Differential Interference Contrast Microscopy
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Imaging Biological Samples with Optical Microscopy
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

