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Mouse Adrenal Chromaffin Cell Isolation
Published on: January 5, 2007
Mouse adrenal chromaffin cell isolation
Aaron Kolski-Andreaco1, Haijiang Cai, D Spencer Currle
1Department of Physiology and Biophysics, University of California, Irvine, CA, USA. aaron.kolski-andreaco@jove.com
Journal of Visualized Experiments : Jove
|October 3, 2008
Abstract:
Adrenal medullary chromaffin cell culture systems are extremely useful for the study of excitation-secretion coupling in an in vitro setting. This protocol illustrates the method used to dissect the adrenals and then isolate the medullary region by stripping away the adrenal cortex. The digestion of the medulla into single chromaffin cells is then demonstrated.
