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Stimuli-activated drug delivery systems are designed to release drugs in response to specific physical, chemical, or biological stimuli. These systems often utilize hydrogels—three-dimensional, hydrophilic polymer networks capable of swelling in aqueous environments and retaining significant fluid volumes. Upon exposure to particular stimuli, these hydrogels undergo structural transitions that allow the embedded drug to be released. Due to this adaptive behavior, such systems are also called...
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Related Experiment Video

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Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
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A novel reversible pH-triggered release immobilized enzyme system.

Lili Gai1, Daocheng Wu

  • 1Key Laboratory of Biomedical Information Engineering of Education Ministry, School of Life Science and Technology, Xi'an Jiaotong University, Xi'an 710049, China.

Applied Biochemistry and Biotechnology
|October 3, 2008
PubMed
Summary

This study developed a novel pH-triggered enzyme release system using hydrogel microspheres. The system enables efficient enzyme immobilization and recovery, offering a promising solution for biotechnology applications.

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Area of Science:

  • Biotechnology
  • Materials Science
  • Enzyme Engineering

Background:

  • Enzyme recovery and reuse are critical in industrial biotechnology.
  • Developing efficient and reusable immobilized enzyme systems remains a challenge.
  • Hydrogel-based systems offer potential for controlled enzyme release.

Purpose of the Study:

  • To develop a novel immobilized enzyme system with reversible pH-triggered release.
  • To investigate the immobilization and release characteristics of enzymes using poly(acrylic acid/N,N'-methylene-bisacryl-amide) hydrogel microspheres.
  • To evaluate the efficiency of enzyme loading, release, and reusability.

Main Methods:

  • Preparation of poly(acrylic acid/N,N'-methylene-bisacryl-amide) hydrogel microspheres.
  • Immobilization of bovine serum albumin and trypsin using gel entrapment and adsorption methods.
  • Characterization of microspheres using optical microscopy, particle size analyzer, and FTIR.
  • Investigation of pH-triggered enzyme release under varying conditions (pH, time, microsphere quantity).

Main Results:

  • Microspheres were spherical with diameters between 3.8-6.6 microm.
  • Adsorption method achieved higher loading efficiency (56.2%) for BSA compared to gel entrapment (93.9%).
  • pH-triggered release occurred above pH 6.0, with high release efficiency (95.0%) for adsorbed protein.
  • Immobilized trypsin showed 77.2% loading efficiency and 91.6% release efficiency at pH 8.0, with 63.3% retained activity.
  • Released trypsin could be reloaded with 51.5% efficiency.

Conclusions:

  • The novel hydrogel microsphere system demonstrates effective reversible pH-triggered enzyme release.
  • The adsorption method is superior for enzyme immobilization in this system.
  • This immobilized enzyme system presents a viable alternative for enzyme recovery in biotechnology.
  • The system's reusability and controlled release properties are advantageous for industrial applications.