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Updated: Jun 29, 2026
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
Published on: November 4, 2016
Identification of Staphylococcus spp. by PCR-restriction fragment length polymorphism analysis of dnaJ gene
Tomasz Hauschild1, Srdjan Stepanovic
1Department of Microbiology, Institute of Biology, University of Bialystok, Bialystok, Poland. thausch@uwb.edu.pl
Abstract:
A PCR-restriction fragment length polymorphism (RFLP) analysis method that analyzes a part of the dnaJ gene was designed for the rapid and accurate identification of Staphylococcus spp. XapI or Bsp143I digestion of the PCR-generated products rendered distinctive RFLP patterns that allowed 41 reference species and subspecies to be identified with a high degree of specificity. The novel method was validated by the identification of 23 clinical staphylococcal strains, and the results were compared with those obtained by other genotypic identification methods. A 100% concordance of the results was shown. Therefore, PCR-RFLP analysis of the dnaJ gene is proposed as a reliable and reproducible method for the identification of Staphylococcus spp.
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