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Related Experiment Video

Updated: Jun 29, 2026

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

Published on: February 27, 2019

Thymus exosomes-like particles induce regulatory T cells.

Gui-Jun Wang¹, Yuelong Liu, Aijian Qin

¹Division of Clinical Immunology and Rheumatology, Department of Medicine, University of Alabama at Birmingham, Birmingham, AL 35294, USA.

Journal of Immunology (Baltimore, Md. : 1950)

|October 4, 2008

Summary

Thymic exosome-like particles (ELPs) promote the development of regulatory T (Treg) cells in peripheral tissues. These thymic ELPs, potentially involving TGF-beta, are crucial for Treg cell generation and immune regulation.

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Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

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Published on: August 13, 2013

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Last Updated: Jun 29, 2026

Determination of Regulatory T Cell Subsets in Murine Thymus, Pancreatic Draining Lymph Node and Spleen Using Flow Cytometry

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Published on: February 27, 2019

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation

Published on: August 13, 2013

Area of Science:

Immunology
Cell Biology
Extracellular Vesicles

Background:

Exosomes mediate intercellular communication.
The thymus is central to T cell development and immune tolerance.

Purpose of the Study:

To investigate the role of thymic exosome-like particles (ELPs) in regulatory T (Treg) cell induction.
To determine the involvement of TGF-beta in thymic ELP-mediated Treg generation.

Main Methods:

Isolation and characterization of thymic ELPs.
In vitro and in vivo assays for Treg cell induction and function.
Neutralization of TGF-beta in thymic ELPs.

Main Results:

Thymic ELPs induce Foxp3(+) Treg cell development in the lung and liver.
Thymic ELPs convert CD4(+)CD25(-) T cells into Tregs.
Induced Tregs suppress T cell proliferation.
TGF-beta neutralization partially inhibits thymic ELP-induced Treg generation.

Conclusions:

Thymic ELPs are key mediators in the induction of Foxp3(+) Treg cells.
TGF-beta within thymic ELPs appears necessary for peripheral Treg generation.