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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Fluorescein pattern interpretation in keratoconus.

Barbara Fink1, Cynthia Heard, Jeff Schafer

  • 1The Ohio State University College of Optometry, 320 West Tenth Avenue, Columbus, OH 43210-1240, USA. fink.4@osu.edu

Optometry and Vision Science : Official Publication of the American Academy of Optometry
|October 4, 2008
PubMed
Summary
This summary is machine-generated.

Clinician accuracy in interpreting contact lens fluorescein patterns for keratoconus does not depend on disease severity. Many factors influence interpretation, not just the stage of keratoconus.

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Area of Science:

  • Ophthalmology
  • Optometry
  • Corneal Disease

Background:

  • Contact lens fitting is crucial for managing keratoconus.
  • Fluorescein pattern assessment is a common method for evaluating contact lens fit.
  • The influence of keratoconus severity on the accuracy of this assessment is not well understood.

Purpose of the Study:

  • To evaluate if keratoconus disease severity affects the accuracy of clinician interpretation of contact lens fluorescein patterns.
  • To determine the relationship between keratoconus severity and the precision of ranking contact lens base curves using fluorescein patterns.

Main Methods:

  • Two clinicians assessed fluorescein patterns on 111 eyes across mild, moderate, and severe keratoconus groups.
  • Clinicians ranked six contact lenses based on fluorescein patterns, from flattest to steepest.
  • Accuracy was measured by percentage of correctly ranked lenses and sum of squared differences from actual base curve values.

Main Results:

  • No statistically significant relationship was found between keratoconus severity and the percentage of correctly ranked contact lenses (p > 0.15).
  • Accuracy of contact lens base curve ranking, as assessed by the sum of squared differences, was not related to keratoconus severity (p > 0.15).

Conclusions:

  • Keratoconus disease severity does not appear to impact the accuracy of contact lens fluorescein pattern interpretation by clinicians.
  • Accurate interpretation relies on a complex interplay of factors including the fluorescein, tears, cornea, contact lens, external forces, and the clinician's technique.