Related Experiment Video
Updated: Jun 29, 2026

The WinCF Model - An Inexpensive and Tractable Microcosm of a Mucus Plugged Bronchiole to Study the Microbiology of Lung Infections
Published on: May 8, 2017
[Involvement of bacterial microflora in etiopathogenesis of chronic sinusitis]
Abstract:
We used the method of amplification of RNA 16S genes in combination with discriminating hybridization of amplification products with species-specific probes in DNA-chip format to identify bacterial flora in patients with chronic sinusitis. A total of 34 samples from 33 patients suffering from chronic sinusitis have been tested. Biopsy specimens of sinus mucosa served as material for sinus microflora studies in the absence of sinusitis exacerbation. It was found that in the exacerbation-free period the samples contained no bacterial RNA 16S while human RNA was isolated from all the samples. Moreover, we isolated and typed fragments of 16S RNA E.fecalis genes from tested in parallel sample of the wall of the oroantral fistula. This confirmed significance and effectiveness of the above method. We came to the conclusion that sinus mucosa is sterile in chronic hyperplastic sinusitis.
Related Concept Videos
Microbiota of the Respiratory Tract
Chronic Pharyngitis
Etiology
It often arises from persistent viral or bacterial infections affecting sinuses and tonsils.
Additional contributing factors include inadequate dental hygiene, mouth breathing, recurring tonsillitis, allergic rhinitis, laryngopharyngeal reflux, and exposure to smoke, chemicals, and other environmental pollutants. Allergic reactions to pollen, mold, and pet dander, chronic cough, excessive voice usage,...
Microbiome of the Eye
The Oral Microbiota
Bacterial Meningitis II: Pathophysiology
Dysbiosis of the Gut Microbiota

