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Related Experiment Video

Updated: Jun 29, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

Epstein-Barr nuclear antigen 1 contributes to nasopharyngeal carcinoma through disruption of PML nuclear bodies.

Nirojini Sivachandran1, Feroz Sarkari, Lori Frappier

  • 1Department of Molecular Genetics, University of Toronto, Toronto, Ontario, Canada.

Plos Pathogens
|October 4, 2008
PubMed
Summary

Epstein-Barr virus (EBV) protein EBNA1 disrupts PML nuclear bodies, promoting cancer cell survival. This EBNA1 function is crucial for nasopharyngeal carcinoma development by impairing DNA repair and apoptosis.

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An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
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Published on: March 30, 2018

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Last Updated: Jun 29, 2026

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09:14

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An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
09:43

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Published on: March 30, 2018

Area of Science:

  • Oncology
  • Virology
  • Molecular Biology

Background:

  • Latent Epstein-Barr virus (EBV) infection is linked to nasopharyngeal carcinoma (NPC).
  • The viral EBNA1 protein maintains EBV episomes during latent infection.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which EBV latent infection drives NPC development.
  • To investigate the role of the EBNA1 protein in cellular processes relevant to cancer.

Main Methods:

  • Examined EBNA1's effect on PML nuclear bodies in native and exogenous expression systems.
  • Assessed EBNA1 interaction with PML isoforms and USP7.
  • Evaluated the impact of EBNA1 on p53 activation, DNA repair, and apoptosis.

Main Results:

  • EBNA1 disrupts PML nuclear bodies, causing PML protein loss.
  • EBNA1 localizes to PML bodies and interacts with a PML isoform, requiring USP7 binding.
  • EBNA1 expression impairs p53 activation, DNA repair, and apoptosis, enhancing cell survival after DNA damage.

Conclusions:

  • EBNA1 plays a significant role in NPC development.
  • EBNA1-mediated disruption of PML nuclear bodies contributes to cancer progression by promoting survival of damaged cells.