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Published on: June 7, 2024
Stress response of Nidula niveo-tomentosa to UV-A light
Daniela E Taupp1, Manfred Nimtz, Ralf G Berger
1Zentrum Angewandte Chemie, Institut für Lebensmittelchemie der Leibniz Universitiit Hannover, Wunstorfer Strasse 14, D-30453 Hannover, Germany.
Abstract:
Exposition to UV-A light stimulated the growth and synthesis of raspberry ketone in submerged cultures of the basidiomycete Nidula niveotomentosa. To investigate the fungus' response to UV-A light differentially expressed proteins were identified by means of 2D-electrophoresis. Light induced proteins were de novo sequenced by ESI-MS/MS spectrometry, and the encoding nucleotide sequences were cloned from cDNA or genomic DNA. The spectrum of UV-A light-induced proteins comprised several stress-related proteins including a catalase, heat-shock proteins, glutathione S-transferases and proteasomes. In addition, growth-related enzymes of the citric cycle were found to be up-regulated as a response to irradiation with UV-A.
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