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Casein kinase I in bovine sperm: purification and characterization
P S Chaudhry1, P A Newcomer, E R Casillas
1Department of Chemistry, New Mexico State University, Las Cruces 88003.
Biochemical and Biophysical Research Communications
|August 30, 1991
Abstract:
A highly purified preparation of sperm casein kinase I was obtained by sequential chromatography with phosphocellulose, gel filtration on sephacryl S-300, Affi-gel blue and DEAE-Cellulose. The chromatographic behavior and properties of the enzyme suggest that the sperm enzyme is similar to casein kinase I from other tissues. Antibodies against calf thymus casein kinase I cross-react with the sperm enzyme. A special feature of the sperm enzyme is that the activity is stimulated by spermine.