Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development and validation of a prediction model for tuberculous peritoneal effusion.

Frontiers in medicine·2026

Same author

Finite element-guided optimization of personalized vacuum bell devices for pectus excavatum: an exploratory retrospective clinical study.

Pediatric surgery international·2026

Same author

Delta radiomics for predicting early radiation-induced lung injury after thoracic radiotherapy: a retrospective paired-CT study.

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]·2026

Same author

Non-caveolar caveolin-1 in retinal Müller glia promotes innate immune responses.

The Journal of biological chemistry·2026

Same author

Development of a machine learning-based prediction model for hypothyroidism-associated delirium in elderly hypothyroid patients in the intensive care unit.

BMC geriatrics·2026

Same author

Non-high-density lipoprotein cholesterol to high-density lipoprotein cholesterol ratio is associated with cardiovascular outcomes after percutaneous coronary intervention in patients with type 2 diabetes.

Frontiers in endocrinology·2026

Same journal

Metal-Organic Framework Multizyme Colloids with Joint Antioxidant and Protease Function.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Morphology Engineering of Co<sub>3</sub>O<sub>4</sub> via Cetyltrimethylammonium Bromide-Mediated ZIF-67 Synthesis for Efficient Photo-Assisted Electrooxidation of Methanol.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Speciation of Silanol Groups on Commercial Precipitated Silicas via IR Spectroscopy.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Regenerable PVA Hydrogel-Functionalized Optical Fiber Sensor for Ultra-Trace Detection of Berberine Hydrochloride.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Hydrogen Plasma-Driven Surface Defect Engineering of ZnO Nanorods: Correlating Electronic Structure and Photoelectrochemical Performance.

Langmuir : the ACS journal of surfaces and colloids·2026

Same journal

Cooperative Self-Assembly of Nanoparticle-Encapsulating Hybrid Protein Cages.

Langmuir : the ACS journal of surfaces and colloids·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 29, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

Cationic conjugated polyelectrolyte/molecular beacon complex for sensitive, sequence-specific, real-time DNA

Xuli Feng¹, Xinrui Duan, Libin Liu

¹Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, PR China.

Langmuir : the ACS Journal of Surfaces and Colloids

|October 7, 2008

Summary

This summary is machine-generated.

A novel fluorescence method enables sensitive, real-time DNA detection at room temperature. This technique utilizes a conjugated polyelectrolyte and molecular beacon system to accurately identify DNA sequences and single nucleotide mismatches.

More Related Videos

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Related Experiment Videos

Last Updated: Jun 29, 2026

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor

Published on: March 21, 2018

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

DNAzyme 10-23 - Based Nanomachines for Nucleic Acid Recognition

Published on: February 9, 2024

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Area of Science:

Biochemistry
Analytical Chemistry
Materials Science

Background:

Developing sensitive and selective DNA detection methods is crucial for diagnostics and research.
Existing methods may lack real-time capabilities, require specific temperatures, or are costly.
Molecular beacons offer excellent mismatch discrimination, while conjugated polyelectrolytes provide efficient light harvesting.

Purpose of the Study:

To develop a novel, room-temperature fluorescence method for sensitive and selective DNA detection.
To integrate the mismatch discrimination of molecular beacons with the light-harvesting properties of conjugated polyelectrolytes.
To achieve economical and real-time DNA detection in aqueous solutions.

Main Methods:

A probe solution was designed containing a cationic conjugated polyelectrolyte (PFP-NMe3+), a fluorescein-labeled molecular beacon (DNA P-Fl), and ethidium bromide (EB).
Electrostatic interactions between DNA P-Fl and PFP-NMe3+ facilitated initial Förster Resonance Energy Transfer (FRET).
Upon target DNA binding, DNA P-Fl formed a duplex, allowing EB intercalation and initiating a two-step FRET process (PFP-NMe3+ to DNA P-Fl, then to EB).

Main Results:

The system demonstrated sensitive and selective DNA detection in aqueous solution through observable changes in fluorescein or EB emission.
The method operates effectively at room temperature, offering a practical advantage.
Single nucleotide mismatches were clearly detected as they inhibited the conformational transition required for the two-step FRET process.

Conclusions:

The developed fluorescence method offers a sensitive, selective, economical, and real-time approach for DNA detection.
The combination of conjugated polyelectrolytes and molecular beacons provides a robust platform for nucleic acid analysis.
This technique holds promise for various applications requiring rapid and accurate DNA identification, including diagnostics and genetic analysis.