Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

GLP-1R and GIPR crosstalk modulates insulinotropic signaling pathways.

Cell chemical biology·2026
Same author

Proteomic analysis of dental enamel from 20 Homo naledi individuals shows no male markers.

Cell·2026
Same author

Methicillin treatment reveals that FtsZ phosphorylation influences the cell division of <i>Streptococcus pneumoniae</i>.

PNAS nexus·2026
Same author

Response to Comment on "Deep Proteogenomics of a Photosynthetic Cyanobacterium".

Journal of proteome research·2026
Same author

Deciphering cytokine-driven ADP-ribosylation signaling networks via Af1521-based mass spectrometry analysis of labile Glu/Asp-linkages.

Nature communications·2026
Same author

The proteomics and phosphoproteomics landscape of melanoma under T cell attack.

Cell reports. Medicine·2026

Related Experiment Video

Updated: Jun 29, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

Global and site-specific quantitative phosphoproteomics: principles and applications.

Boris Macek1, Matthias Mann, Jesper V Olsen

  • 1Max Planck Institute for Biochemistry, Martinsried, Germany.

Annual Review of Pharmacology and Toxicology
|October 7, 2008
PubMed
Summary

Phosphorylation, a key cell process, is analyzed using advanced proteomics. This review covers methods for studying phosphoproteomes, aiding cell signaling research and drug discovery.

More Related Videos

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Related Experiment Videos

Last Updated: Jun 29, 2026

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae
15:41

Quantitative Phosphoproteomics in Fatty Acid Stimulated Saccharomyces cerevisiae

Published on: October 12, 2009

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer
12:23

Phosphopeptide Enrichment Coupled with Label-free Quantitative Mass Spectrometry to Investigate the Phosphoproteome in Prostate Cancer

Published on: August 2, 2018

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
09:10

A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes

Published on: May 22, 2018

Area of Science:

  • Biochemistry and Molecular Biology
  • Proteomics
  • Cell Signaling

Background:

  • Protein phosphorylation is a crucial posttranslational modification regulating cellular processes.
  • Dysregulated signaling pathways are implicated in cancer and other diseases, making protein kinases significant drug targets.
  • Traditional methods using phosphorylation-specific antibodies offer targeted analysis, but lack global scope.

Purpose of the Study:

  • To review current enrichment strategies and mass spectrometry methods for phosphopeptide analysis.
  • To describe quantitative phosphoproteomics techniques for analyzing large-scale phosphorylation changes.
  • To highlight the application of phosphoproteomics in cell signaling and drug target discovery.

Main Methods:

  • Phosphopeptide enrichment techniques.
  • Mass spectrometry-based fragmentation and analysis of phosphopeptides.
  • Quantitative proteomics methods for large-scale phosphorylation site analysis.

Main Results:

  • Advances in proteomics enable comprehensive analysis of entire phosphoproteomes.
  • Quantitative methods allow assessment of thousands of phosphorylation sites in a single experiment.
  • Phosphoproteomics is applicable to diverse research areas, including bacterial signaling.

Conclusions:

  • Emerging phosphoproteomics technologies offer robust and comprehensive approaches to study cell signaling.
  • These methods are vital for understanding disease mechanisms and identifying novel drug targets.
  • The scope of phosphoproteomics extends beyond eukaryotic cell signaling to other biological systems.