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Updated: Jun 29, 2026
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Published on: July 23, 2017
Microarray analysis of acaricide-inducible gene expression in the southern cattle tick, Rhipicephalus (Boophilus)
L Saldivar1, F D Guerrero, R J Miller
1USDA-ARS Knipling-Bushland US Livestock Insects Research Laboratory, Kerrville, TX 78028, USA.
Abstract:
Acaricide-inducible differential gene expression was studied in larvae of Rhipicephalus (Boophilus) microplus using a microarray-based approach. The acaricides used were: coumaphos, permethrin, ivermectin, and amitraz. The microarrays contained over 13 000 probes, having been derived from a previously described R. microplus gene index (BmiGI Version 2; Wang et al., 2007). Relative quantitative reverse transcriptase-PCR, real time PCR, and serial analysis of gene expression data was used to verify microarray data. Among the differentially expressed genes with informative annotation were legumain, glutathione S-transferase, and a putative salivary gland-associated protein.
