Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 29, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Automated study of ligand-receptor binding using solid-phase microextraction.

Dajana Vuckovic1, Janusz Pawliszyn

  • 1Department of Chemistry, University of Waterloo, Waterloo, Ontario, Canada.

Journal of Pharmaceutical and Biomedical Analysis
|October 7, 2008
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual Acidic pH-Responsive Post-Crosslinked E-Spun Nanofibrous Scaffolds Exhibiting Enhanced Disassembly and Release for Localized Cancer Treatment.

Advanced healthcare materials·2026
Same author

<i>In Vivo</i> Negligible Depletion SPME for the Determination of Free and Total Concentrations of Anandamide and 2-Arachidonoylglycerol in the Brain of a Parkinson's Disease Rat Model.

Analytical chemistry·2026
Same author

Defects in auxiliary fuel oxidation and mitochondrial pyruvate transport mark transition to overt heart failure in Tgαq*44 mice.

Journal of translational medicine·2026
Same author

High-throughput screening of per- and polyfluoroalkyl substances in human plasma using biocompatible solid-phase microextraction coupled with mass spectrometry via microfluidic open interface.

Analytica chimica acta·2026
Same author

Comprehensive analysis of exhaled breath VOCs using GC-MS and GC×GC-TOF-MS: a comparative platform evaluation with TFME and NTD sampling for free and total concentrations.

Analytical and bioanalytical chemistry·2025
Same author

Preoperative untargeted SPME-LC/HRMS-based metabolomic profiling in cardiac surgical patients identified prognostic biomarkers for postoperative outcomes.

Analytica chimica acta·2025
Same journal

Analyzing the impact of ionizable lipid identity, purity, and stability on lipid nanoparticle performance.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Application of two-dimensional liquid chromatography as a complementary technique to circular dichroism spectroscopy and high-resolution mass spectrometry for the characterization of GalNAc-siRNA conjugates.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

The transfer of per- and polyfluoroalkyl substances (PFAS) from mother to child: Comparison between maternal and cord blood in an Italian cohort.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

UHPLC/Q-TOF-MS-based blood-component profiling and multi-omics analysis reveal potential protective mechanisms of Shenzhuo Formula against diabetic kidney disease.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Multi-center study of the recombinant cascade reagent (kinetic chromogenic assay) as an alternative method for bacterial endotoxin testing: Method validation, product suitability, and consistency evaluation with limulus amebocyte lysate.

Journal of pharmaceutical and biomedical analysis·2026
Same journal

Simultaneous enantioselective separation of 2-, 3- and 4-chloromethcathinones using supercritical fluid chromatography-tandem mass spectrometry and its application to human oral fluid samples.

Journal of pharmaceutical and biomedical analysis·2026
See all related articles

This study introduces automated solid-phase microextraction (SPME) for ligand-receptor binding analysis. The novel method accurately determined diazepam binding to human serum albumin, offering a faster, automated alternative for complex sample analysis.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Pharmacology

Background:

  • Ligand-receptor binding studies are crucial for understanding biological processes and drug interactions.
  • Traditional methods can be time-consuming and labor-intensive.
  • Solid-phase microextraction (SPME) offers a potential for automation and simplification.

Purpose of the Study:

  • To develop and validate a novel automated solid-phase microextraction (SPME) system for ligand-receptor binding analysis.
  • To evaluate the performance of the automated SPME system using diazepam binding to human serum albumin as a model.
  • To demonstrate the applicability of the method for complex biological samples.

Main Methods:

  • Development of a multi-well plate-based automated SPME system for parallel sample preparation.

More Related Videos

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Related Experiment Videos

Last Updated: Jun 29, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

  • Coupling of automated SPME with liquid chromatography-tandem mass spectrometry (LC-MS/MS).
  • Determination of equilibrium time and application of fibre constant calibration for data normalization.

    • Main Results:

    • The automated SPME system enabled parallel preparation of up to 96 samples in a single experiment.
    • Equilibrium time for diazepam binding to human serum albumin was determined to be 30 minutes.
    • A binding constant of 9.1 x 10^5 ± 3 x 10^5 L/mol was obtained, consistent with literature values.

    Conclusions:

    • The automated SPME method provides a simple, fast, and fully automated approach for ligand-receptor binding studies.
    • The method is suitable for direct analysis of complex samples, including plasma-protein and whole blood binding.
    • This technology has the potential to significantly advance drug discovery and pharmacokinetic studies.