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Related Concept Videos

High-Performance Liquid Chromatography: Elution Process01:05

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
High-Performance Liquid Chromatography: Instrumentation00:57

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.
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High-Performance Liquid Chromatography: Introduction

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High-Performance Liquid Chromatography: Types of Detectors01:15

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Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
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External mass transfer in high performance liquid chromatography systems.

Kanji Miyabe1, Motoki Ando, Nobuho Ando

  • 1Graduate School of Science and Engineering for Research, University of Toyama, 3190, Gofuku, Toyama 930-8555, Japan.

Journal of Chromatography. A
|October 7, 2008
PubMed
Summary

This study investigated external mass transfer in reversed-phase High-Performance Liquid Chromatography (HPLC). Conventional correlations accurately estimate mass transfer coefficients even for micrometer-sized particles.

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Published on: May 20, 2013

Area of Science:

  • Analytical Chemistry
  • Separation Science
  • Chemical Engineering

Background:

  • External mass transfer is crucial for chromatographic separation efficiency.
  • Understanding mass transfer in High-Performance Liquid Chromatography (HPLC) is vital for optimizing separation.
  • Previous studies established correlations for mass transfer in packed beds, but applicability to micrometer-sized particles needed verification.

Purpose of the Study:

  • To investigate external mass transfer in reversed-phase HPLC using non-porous C(18)-silica gel particles.
  • To determine the external mass transfer coefficient (k(f)) under varying flow velocities and temperatures.
  • To validate the applicability of conventional literature correlations for micrometer-scale particles.

Main Methods:

  • Pulse response experiments were conducted in a High-Performance Liquid Chromatography (HPLC) system.
  • A column packed with 18-micrometer non-porous C(18)-silica gel particles was used.
  • The moment method was employed to analyze elution peak moments and determine the external mass transfer coefficient (k(f)).

Main Results:

  • The Sherwood number dependence on Reynolds and Schmidt numbers aligned with conventional correlations.
  • The derived exponent for these dimensionless parameters ranged from 0.28 to 0.41.
  • The Kataoka equation showed a low mean square deviation (0.21) for estimated k(f) values.

Conclusions:

  • Conventional correlations are suitable for estimating external mass transfer coefficients (k(f)) in reversed-phase HPLC.
  • These correlations remain valid even when using micrometer-sized particles.
  • The findings support the use of established models for predicting mass transfer in advanced chromatographic systems.