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Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
Published on: July 4, 2016
An ESR characterization of micelles and vesicles formed in aqueous decanoic acid/sodium decanoate systems using
Branka Dejanović1, Krunoslav Mirosavljević, Vesna Noethig-Laslo
1Department of Physical Chemistry, Ruder Bosković Institute, Zagreb, Croatia.
Abstract:
Aqueous decanoic acid/sodium decanaote systems were studied as a function of pH and concentration, up to 0.3 M decanoic acid/sodium decanoate, by electron spin resonance (ESR) spectroscopy using three different amphiphilic spin labels. The distribution of the spin labels between vesicles and micelles as well as their dynamic properties were determined by quantitative analysis of the ESR spectra using two novel simulation software packages. Rotational correlation time of the labels in micelles was found to increase with decreasing pH, with substantial increase in the region where vesicles were formed (7.8
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