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PCR01:32

PCR

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Related Experiment Video

Updated: Jun 29, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

Predicting Salmonella enterica serotypes by repetitive sequence-based PCR.

Mark G Wise1, Gregory R Siragusa, Jodie Plumblee

  • 1Bacterial Barcodes, Inc. 425 River Rd., Athens, GA 30602, USA.

Journal of Microbiological Methods
|October 7, 2008
PubMed
Summary

Repetitive extragenic palindromic sequence-based PCR (rep-PCR) offers a rapid method for Salmonella serotyping in poultry. This DNA fingerprinting technique shows promise for faster classification compared to traditional serological methods.

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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
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Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

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Last Updated: Jun 29, 2026

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium
12:18

An Allelotyping PCR for Identifying Salmonella enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium

Published on: July 22, 2011

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples
06:12

Quasi-metagenomic Analysis of Salmonella from Food and Environmental Samples

Published on: October 25, 2018

Area of Science:

  • Microbiology
  • Molecular Biology
  • Food Safety

Background:

  • Accurate Salmonella serotyping is crucial for food safety and epidemiology.
  • Traditional serological methods can be time-consuming and challenging for differentiating closely related serotypes.

Purpose of the Study:

  • To evaluate the DiversiLab System, a semi-automated rep-PCR method, for rapid Salmonella serotype determination in poultry.
  • To assess the utility of rep-PCR in creating a DNA fingerprint library for Salmonella serotype classification.

Main Methods:

  • A DNA fingerprint library was created using 216 Salmonella isolates from poultry.
  • A blinded set of 44 poultry isolates were fingerprinted and compared against the library using Pearson correlation coefficient.
  • rep-PCR results were compared with standard serological typing results.

Main Results:

  • 28 out of 44 isolates were correctly assigned a serotype designation.
  • rep-PCR showed potential in distinguishing between closely related serotypes like Hadar and Istanbul, which are difficult for traditional methods.
  • Six isolates not present in the library were correctly identified as unknown, and two showed discrepancies requiring library expansion.

Conclusions:

  • The DiversiLab System using rep-PCR is a promising tool for rapid Salmonella serotype classification in poultry.
  • This method offers a significant time advantage over traditional serological assays.
  • Further library expansion is recommended to increase the utility and accuracy of the rep-PCR method for broader Salmonella serotype identification.