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Related Concept Videos

Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
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T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
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When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
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Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
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Relationship between CD107a expression and cytotoxic activity.

Esin Aktas1, Umut Can Kucuksezer, Sema Bilgic

  • 1Department of Immunology, Institute of Experimental Medicine (DETAE), Istanbul University, Vakif Gureba Caddesi, Sehremini, 34393 Istanbul, Turkey.

Cellular Immunology
|October 7, 2008
PubMed
Summary
This summary is machine-generated.

CD107a expression is a sensitive marker for cytotoxic activity in Natural Killer (NK) cells and CD8+ T cells. Interleukin-2 (IL-2) stimulation increases CD107a levels, correlating with enhanced cell-mediated killing of tumor cells.

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Published on: August 8, 2012

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Natural Killer (NK) cells are crucial for innate immunity against tumors and infections.
  • CD107a (LAMP-1) is recognized as a potential marker for the degranulation of NK cells and activated CD8+ T cells.

Purpose of the Study:

  • To investigate the relationship between CD107a expression, cytokine secretion, and cytotoxic activity in CD56+ NK cells, CD8+ T cells, and lymphocytes.
  • To determine the effect of various stimuli on these immune cell functions.

Main Methods:

  • Isolation of effector cells from peripheral blood mononuclear cells (PBMCs) of healthy donors.
  • Utilized the K562 cell line as a target for cytotoxicity assays.
  • Stimulated cells with Interleukin-2 (IL-2) and measured CD107a expression, cytokine secretion, and cytotoxic activity.

Main Results:

  • IL-2 stimulation significantly increased CD107a expression in CD56+ NK cells, CD8+ T cells, and lymphocytes.
  • In NK cells, increased CD107a expression following IL-2 stimulation was directly parallel to enhanced cytotoxic activity.
  • Cytokine secretion patterns were also analyzed in conjunction with CD107a expression and cytotoxicity.

Conclusions:

  • CD107a expression serves as a sensitive indicator for assessing the cytotoxic activity of immune cells, particularly NK cells.
  • IL-2 is an effective stimulus for enhancing both CD107a expression and cytotoxic function in these cells.
  • The findings support the utility of CD107a as a biomarker in immune monitoring and therapeutic evaluations.