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Output grating couplers on planar optical waveguides as direct immunosensors
W Lukosz1, D Clerc, P M Nellen
1Optics Laboratory, Swiss Federal Institute of Technology, Zürich.
Biosensors & Bioelectronics
|January 1, 1991
Summary
This study shows integrated optical output grating couplers can detect antigen-antibody binding in real-time. This biosensing method quantifies human immunoglobulin G (h-IgG) interactions for potential diagnostic applications.
Area of Science:
- Biophotonics
- Biosensing
- Immunochemistry
Background:
- Direct immunosensing offers label-free detection of biomolecular interactions.
- Integrated optical devices provide a platform for miniaturized and sensitive biosensors.
Purpose of the Study:
- To demonstrate the feasibility of using integrated optical output grating couplers for direct immunosensing.
- To monitor antigen (Ag) adsorption and antibody (Ab) binding in real-time.
- To quantify the optical properties and surface coverage of adsorbed layers.
Main Methods:
- Utilized integrated optical output grating couplers for real-time monitoring.
- Investigated the adsorption of human immunoglobulin G (h-IgG) and rabbit anti-h-IgG.
- Studied the adsorption of avidin.
- Determined refractive indices, thicknesses, and surface coverages of adsorbed layers.
Main Results:
- Successfully demonstrated real-time monitoring of antigen adsorption and antibody binding.
- Quantified the formation of the antigen-antibody (Ag-Ab) immuno-complex.
- Determined optical properties and surface coverage of adsorbed layers, including h-IgG and avidin.
Conclusions:
- Integrated optical output grating couplers are feasible for direct immunosensing.
- The method allows for real-time quantification of specific biomolecular interactions.
- This approach holds potential for label-free biosensing applications.