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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.
Telomeres and Telomerase02:41

Telomeres and Telomerase

In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded DNA.

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Updated: Jun 29, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Canis familiaris telomerase reverse transcriptase undergoes alternative splicing.

Katerina Angelopoulou1, Michael Zavlaris, Nikolaos Papaioannou

  • 1Laboratory of Biochemistry and Toxicology, School of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, 54124, Greece. kangelop@vet.auth.gr

Mammalian Genome : Official Journal of the International Mammalian Genome Society
|October 7, 2008
PubMed
Summary

Alternative splicing of canine telomerase reverse transcriptase (dogTERT) was investigated. Five novel dogTERT variants were identified, all encoding truncated proteins, suggesting altered telomerase activity in canine cells.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cancer Research

Background:

  • Telomerase is crucial for cell proliferation and cancer.
  • Telomerase reverse transcriptase (TERT) is the catalytic subunit.
  • Alternative splicing regulates human TERT expression.

Purpose of the Study:

  • To investigate alternative splicing in canine TERT (dogTERT).
  • To determine if dogTERT exhibits sequence similarity to human TERT, supporting its use as a model.
  • To analyze dogTERT expression in canine mammary tissues.

Main Methods:

  • RT-PCR analysis of dogTERT expression in canine mammary tissues.
  • DNA sequencing of PCR products with unexpected sizes.
  • Identification and characterization of alternative splicing variants.

Main Results:

  • Five distinct dogTERT transcripts were identified due to alternative splicing.
  • These variants included insertions (175 bp, 28 bp) and deletions (17 bp, 32 bp) within exons 7 and 8.
  • All identified variants encoded truncated proteins lacking essential reverse transcription motifs.

Conclusions:

  • This is the first report of alternative splicing in dogTERT.
  • Alternative splicing generates non-functional dogTERT variants.
  • These findings provide a basis for studying telomerase regulation in canine normal and cancer cells.