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Updated: Jun 29, 2026

Colonization with Murine pks+ Escherichia coli under Non-Inflammatory Conditions
Published on: March 10, 2026
Cytokine response to Escherichia coli in gnotobiotic pigs
I Splichal1, A Splichalova, I Trebichavsky
1Division of Immunology and Gnotobiology, Academy of Sciences of the Czech Republic, Czechia. splichal@gnotobio.cz
Abstract:
One-week-old-germ-free pigs were inoculated with 10(8) CFU of E.coli bacteria-either commensal 086 strain or virulent 055 strain for 1 d. Bacteria were counted in the small intestine, mesenteric lymph nodes, blood and lungs. The O55 strain reached higher levels in circulation and lungs. IL-8, IL-10 and TNF-alpha concentrations were determined by ELISA in plasma and intestinal washes . No difference in cytokine levels was found between control germ-free pigs and their counterparts associated with commensal O86 strain in spite of its high concentration in the gut and circulation.
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