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Updated: Jun 29, 2026

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Published on: September 15, 2015
[Actinobacterial diversity of a sediment sample from Xiaoerkule Lake]
Tongwei Guan1, Jinyuan Wu, Xiaoyang Zhi
1Key Laboratory of Protection and Utilzation of Biological Resources in Tarim Basin of Xinjiang Production & Construction Corps, Tarim University, Alar 843300, China. guantongweily@163.com
Objective:
The aim of study was to investigate Actinobacterial diversity in Xiaoerkule salt lake, to lay a foundation for furthering to tap.
Methods:
Actinobacterial diversity in this sediment from Xiaoerkule Lake was investigated by culture-independent method and phylogenetic analysis based on 16S rRNA gene sequences. Total DNA of sediment sample was extracted using SDS-CTAB method. The primers for the class Actinobacteria were used for actinobacterial 16S rRNA gene amplification and then a clone library was constructed for the sediment sample.
Results:
Fifty-one clones screened from 160 clones on the basis of Hae III digestion patterns were sequenced, and their sequences were deposited in the GenBank. Clone sequences (52.9%) belonged to Acidimicrobidae and 5 suborders of Actinobacteridae. The other clone sequences (47.1%), which formed one large distinct clade in phylogenetic tree among phylum Actinobacteria, may represent one new suborder or new class.
Conclusion:
There was abundant actinobacterial diversity in the sediment of Xiaoerkule Lake, and the result implied that there were large numbers of unknown actionobacterial groups here.
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