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Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
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[Detecting hematolysis of Enterococcus from sheep].

Lichao Kang1, Sujuan Han, Jingmei Wang

  • 1College ofAnimal Science and technology, Shihezi University, Shihezi 832003, China. klc003@163.com

Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|October 8, 2008
PubMed
Summary

Enterococcus isolates from sheep exhibit varied hemolytic activity, with clinical strains showing beta-hemolysis and alpha-hemolysis, unlike healthy isolates. Genotype analysis revealed the presence of the cylA gene in clinical isolates, differing from phenotypic results.

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Area of Science:

  • Veterinary Microbiology
  • Bacterial Pathogenesis
  • Molecular Diagnostics

Context:

  • Enterococcus species are significant in both human and animal health.
  • Understanding their hemolytic potential is crucial for disease diagnosis and control in sheep.
  • Previous studies have not fully elucidated the hemolytic characteristics of ovine Enterococcus isolates.

Purpose:

  • To characterize the hemolytic activity of Enterococcus isolates from sheep using various assays.
  • To correlate hemolytic phenotypes with the presence of the cylA gene.
  • To differentiate between clinical and healthy isolates regarding hemolysis.

Summary:

  • Plate assay (PA), contact hemolysis (CH), supernatant assay (SA), and culture hemolysis (CLH) were employed to study 11 clinical and 30 healthy sheep Enterococcus isolates.
  • Clinical isolates predominantly showed beta-hemolysis (63.6%) with rabbit blood and alpha-hemolysis (36.4%) with sheep blood, with 63.6% harboring the cylA gene.
  • Healthy isolates displayed lower hemolytic activity, with the cylA gene absent, indicating a genotypic and phenotypic divergence.

Impact:

  • This study provides a comprehensive characterization of Enterococcus hemolysis in sheep.
  • The findings highlight discrepancies between hemolytic phenotype and genotype, aiding in accurate diagnosis.
  • Results contribute to understanding Enterococcus virulence factors in ovine populations.