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DNA Isolation01:34

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

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Related Experiment Video

Updated: Jun 29, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

A method for characterization of total circulating DNA.

Maniesh van der Vaart1, Piet J Pretorius

  • 1School of Biochemistry, North-West University, Potchefstroom Campus, Potchefstroom, South Africa.

Annals of the New York Academy of Sciences
|October 8, 2008
PubMed
Summary
This summary is machine-generated.

Sequencing total circulating DNA reveals uncharacterized genomic sequences and repeats, with potential diagnostic value. Analysis showed more Alu repeats in healthy individuals than cancer patients.

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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
07:37

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

Related Experiment Videos

Last Updated: Jun 29, 2026

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR
07:37

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Circulating DNA in plasma is increasingly studied, but comprehensive sequencing data remains limited.
  • Understanding the origin and function of circulating DNA requires detailed sequence characterization.

Purpose of the Study:

  • To characterize the sequence of total circulating DNA from plasma using cloning and sequencing.
  • To identify novel genomic sequences and repeat elements within circulating DNA.
  • To compare circulating DNA profiles between a healthy individual and a cancer patient.

Main Methods:

  • Isolation of circulating DNA from plasma using various methods.
  • Cloning of isolated DNA into a blunted vector.
  • Sequencing and bioinformatic analysis of cloned DNA fragments.

Main Results:

  • The majority of cloned circulating DNA fragments were approximately 200 bp in length.
  • Sequence analysis identified uncharacterized human genomic sequences and known gene-containing regions.
  • Alu repeat sequences were prevalent, with preliminary findings suggesting higher abundance in healthy individuals compared to cancer patients.
  • Commonly reported circulating DNA genes (e.g., P53, Ras) were not detected.

Conclusions:

  • Cloning and sequencing of free circulating DNA is feasible and provides valuable sequence data.
  • Circulating DNA comprises a diverse range of sequences, including novel genomic elements and repeats.
  • Further characterization of circulating DNA sequences holds promise for diagnostic and prognostic applications.