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Monitoring Dynamic Growth of Retinal Vessels in Oxygen-Induced Retinopathy Mouse Model
Published on: April 2, 2021
Comprehensive gene-expression profile in murine oxygen-induced retinopathy.
1Department of Applied Visual Science, Osaka University Medical School, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
The British Journal of Ophthalmology
|October 8, 2008
Summary
Gene expression patterns in mouse models of retinopathy of prematurity (ROP) closely mirrored clinical progression. Understanding these gene expression changes aids in comprehending ROP pathology.
Area of Science:
- Ophthalmology
- Molecular Biology
- Developmental Biology
Background:
- Oxygen-induced retinopathy (OIR) in mice is a key model for studying retinopathy of prematurity (ROP).
- Understanding the molecular mechanisms underlying OIR is crucial for developing effective treatments for ROP.
Purpose of the Study:
- To investigate the correlation between the clinical course of OIR in mice and their gene-expression patterns.
- To identify key genes and pathways involved in OIR progression.
Main Methods:
- OIR was induced in C57BL/6N mice.
- Retinal flat-mounts were evaluated from postnatal day 12 (P12) to P21.
- Gene expression of 94 selected genes was analyzed daily using RT-PCR with TaqMan low-density array (TLDA) and hierarchical clustering.
Main Results:
- Gene expression patterns showed homology between P12-P13 and P16-P17.
- Inflammation-associated genes were upregulated during maximal avascularity and vasoconstriction (P12-P13), continuing to P21.
- Angiogenesis-associated genes, including VEGF-A and angiopoietin-2, were most upregulated during peak extraretinal neovascularization (P16-P17).
Conclusions:
- The gene-expression pattern in murine OIR strongly correlates with the observed clinical progression.
- These findings enhance the understanding of the pathological processes in retinopathy of prematurity.
