Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A function for the integrin alpha 6 beta 4 in the hemidesmosome.

J C Jones1, M A Kurpakus, H M Cooper

  • 1Cell, Molecular, and Structural Biology Department, Northwestern University Medical School, Chicago, Illinois 60611.

Cell Regulation
|June 1, 1991
PubMed
Summary

Integrins, specifically alpha 6 beta 4, are crucial for hemidesmosome assembly and function in epithelial cells. Blocking this integrin prevents hemidesmosome formation, highlighting its role in cell adhesion and tissue repair.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

State-independent ionic conductivity.

Science (New York, N.Y.)·2025
Same author

People Living with HIV's Worry That the COVID-19 Health Crisis Could Impact Long-Term HIV Care: Lessons From the French Context for Future Disease Epidemics.

Journal of the International Association of Providers of AIDS Care·2025
Same author

Low risk management intervention: Limited impact of remedial tillage on net ecosystem carbon balance at a commercial Miscanthus plantation.

Global change biology. Bioenergy·2024
Same author

Overriding water table control on managed peatland greenhouse gas emissions.

Nature·2021
Same author

Variable pitch hydrodynamic electro-optic gratings utilising bent liquid crystal dimers.

Soft matter·2020
Same author

Sphingosine kinase 1 overexpression induces MFN2 fragmentation and alters mitochondrial matrix Ca<sup>2+</sup> handling in HeLa cells.

Biochimica et biophysica acta. Molecular cell research·2019

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Epithelial cells anchor to connective tissue via hemidesmosomes.
  • Hemidesmosomes are poorly understood biochemically, impacting blistering diseases and wound repair.

Purpose of the Study:

  • To investigate the role of integrin alpha 6 beta 4 in hemidesmosome assembly and function.

Main Methods:

  • Immunoelectron microscopy to localize integrin alpha 6 beta 4.
  • Immunoprecipitation and immunofluorescence in 804G cells.
  • Ultrastructural analysis of cells treated with alpha 6 beta 4 antibodies.

Main Results:

  • Integrin alpha 6 beta 4 localizes to hemidesmosomes.
  • Blocking alpha 6 beta 4 disrupts hemidesmosome formation in cultured cells.

Related Experiment Videos

  • Alpha 6 beta 4 distribution overlaps with hemidesmosomal plaque antigens.

    • Conclusions:

    • Integrin alpha 6 beta 4 plays a critical role in hemidesmosome assembly.
    • This integrin is essential for the adhesive functions of hemidesmosomes.