Physicochemical properties of diheme cytochrome c4 of unknown function from Vibrio parahaemolyticus strain RIMD2210633
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Updated: Jun 29, 2026

Isolation and Characterization of Intact Phycobilisome in Cyanobacteria
Published on: November 10, 2021
Hideharu Akazaki1, Yoshio Futami, Naoya Shibayama
1Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University, Kanagawa, Japan.
Abstract:
To characterize a diheme cytochrome c4 of unknown functional of the Vibrio genus for the first time, the Vibrio parahaemolyticus cytochrome c4 was overexpressed in Escherichia coli periplasm using the endogenous signal sequence. The physicochemical properties of the purified recombinant protein, viz., molecular mass, UV/Vis, and CD spectra, and the redox potentials of the N- and C-terminal domain hemes were determined.
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